The demand for a sustainable supply of critical battery metals, such as Ni, Co, and Cu, is growing. Glycine has recently been employed as a leaching agent to extract these metals from their host materials. Although the leached metals can be recovered through mixed sulphide precipitation, further processing of the sulphide precipitates is necessary to achieve battery-grade standards. This study investigates the sequential separation of Co, Cu, and Ni from alkaline glycine solutions via solvent extraction. The results showed that Cu consistently demonstrated high extraction efficiency, exceeding 95%. Similarly, Co maintained an efficiency above 95%, except at higher glycine concentrations, where it dropped to 80%. In contrast, Ni achieved satisfactory extraction efficiency (>90%) only when the extraction time was extended to 4.0 hours, indicating that Ni extraction is kinetically, or mass transfer limited. While Cu (~100%) and Ni (>80%) could be stripped from LIX 84-IC using 200 g/L H2SO4, Co stripping was negligible. Sequential separation of the three metals was accomplished by selectively extracting Co with Cyanex 272, followed by the extraction of Cu and Ni with LIX 84-IC. Glycine, recognized as a "green" lixiviant for metal extraction in alkaline conditions, effectively minimizes gangue mineral dissolution, addressing both the economic and environmental challenges of conventional high-pressure acid leaching and promoting process sustainability.