The quantitative study on the effect of mass transfer on dynamic interfacial tension and droplet breakage is a critical issue for chemical engineering. Based on previous research in this area, dynamic interfacial tension seems to be the bridge between mass transfer and droplet breakage. In this study, a method is developed to measure the dynamic interfacial tension in a stirred tank. The method is based on the second order oscillation model which describes the relation between breakage frequency and interfacial tension. When breakage frequency is determined by high-speed camera experimentally, the interfacial tension is obtained by least square regression. With this method, the dynamic interfacial tension under mass transfer condition was determined, and the effect of mass transfer flux and mass transfer direction were also investigated. To better understand the dispersion and mass transfer process, a single droplet model combined with computational fluid dynamics simulation was developed to analyze droplet swarm behavior. The effects of impeller rotating speed, initial solute concentration and continuous phase residence time on droplet breakage and mass transfer are well explained. These results are helpful to understand droplet swarm mass transfer process in depth.